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NCJ Number: 237527 Find in a Library
Title: Belgian Canine Population and Purebred Study for Forensics by Improved Mitochondrial DNA Sequencing
Journal: Forensic Science International: Genetics  Volume:6  Issue:1  Dated:January 2012  Pages:108-112
Author(s): Stijn Desmyter; Leonie Gijsbers
Date Published: January 2012
Page Count: 5
Type: Report (Study/Research)
Format: Article
Language: English
Country: Ireland
Annotation: This canine population study examined the mitochondrial DNA of the 208 breed and mixed dogs.
Abstract: In canine population studies for forensics, the mitochondrial DNA is profiled by sequencing the two hyper variable regions, HV1 and HV2 of the control region. In a first effort to create a Belgian population database some samples showed partially poor sequence quality. The authors demonstrated that a nuclear pseudogene was co-amplified with the mtDNA control region. Using a new combination of primers this adverse result was no longer observed and sequencing quality was improved. All former samples with poor sequence data were reanalyzed. Furthermore, the forensic canine population study was extended to 208 breed and mixed dogs. In total, 58 haplotypes were identified, resulting in an exclusion capacity of 0.92. The profile distribution of the Belgian population sample was not significantly different from those observed in population studies of three other countries. In addition to the total population study 107 Belgian registered pedigree dogs of 6 breeds were profiled. Per breed, the obtained haplotypes were supplemented with those from population and purebred studies. The combined data revealed that some haplotypes were more or less prominent present in particular dog breeds. The statistically significant differences in haplotype distribution between breeds and population sample can have consequences on mtDNA databasing and matching probabilities in forensics. (Published Abstract)
Main Term(s): Criminology
Index Term(s): Belgium; Databases; DNA fingerprinting; Foreign criminal justice research; Forensic sciences; Investigative techniques
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