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NCJ Number: 47273 Find in a Library
Title: ANALYSIS OF DRUGS IN BLOOD, BILE, AND TISSUE WITH AN INDIRECT HOMOGENEOUS ENZYME IMMUNOASSAY
Journal: JOURNAL OF FORENSIC SCIENCES  Volume:23  Issue:2  Dated:(APRIL 1978)  Pages:292-303
Author(s): E L SLIGHTOM
Corporate Author: American Soc for Testing and Materials
Promotions Manager
United States of America
Date Published: 1978
Page Count: 12
Sponsoring Agency: American Soc for Testing and Materials
West Conhohocken, PA 19428-2959
Georgia Crime Laboratory
Atlanta, GA 30301
Sale Source: Georgia Crime Laboratory
Toxicology Section
P.O. Box 1456
Atlanta, GA 30301
United States of America
Format: Article
Language: English
Country: United States of America
Annotation: THE EXTENSION OF THE ENZYME MULTIPLIED IMMUNOASSAY TECHNIQUE (EMIT), A DRUG DETECTION METHOD USED PRIMARILY FOR URINE AND SERUM ANALYSIS, TO ANALYSIS OF DRUGS IN BLOOD, BILE, AND TISSUE IS REPORTED.
Abstract: THE EMIT METHODOLOGY HAS BEEN DESCRIBED IN DETAIL ELSEWHERE BY SYVA CORPORATION, THE MANUFACTURER. LYSOZYMES WITH ATTACHED DRUGS WERE SUPPLIED BY THE MANUFACTURER. PROCEDURES FOR DRUG EXTRACTION, INCLUDING REAGENTS, ARE DESCRIBED FOR THE FOLLOWING DRUGS: PHENOBARBITAL, PENTOBARBITAL, MORPHINE SULFATE, CODEINE SULPHATE, HYDROMORPHONE HYDROCHLORIDE, MEPERIDINE HYDROCHLORIDE, COCAINE HYDROCHLORIDE, NORDIAZEPAM, DIAZEPAM, AND PROPOXYPHENE HYDROCHLORIDE. CRITERIA WHICH MUST BE MET IN EXTENDING EMIT TO THE ANALYSIS OF BIOLOGICAL FLUIDS AND TISSUES INCLUDE: THE DRUG MUST BE EXTRACTED; PARTITIONING OF NATIVE LYSOZYMES MUST BE AVOIDED OR LYSOSOMES MUST BE DENATURED BY ORGANIC SOLENT; AND THE PARTITIONING OF THE PRODUCTS OF THE LYSOZYME REACTION OR ANY OTHER INHIBITORS MUST NOT TAKE PLACE. THE DIRECT AND INDIRECT (I.E. THROUGH EXTRACTION FROM WHOLE BLOOD, BILE, OR TISSUE) SENSITIVITIES OF EMIT TO THE DRUGS ANALYZED ARE PRESENTED IN TABULAR FORM. IN GENERAL, INDIRECT EMIT SENSITIVITY IS VERY HIGH FOR MORPHINE, CODEINE, AND HYDROPOMORPHINE, GOOD FOR NORDIAZEPAM AND DIAZEPAM, AND LEAST SENSITIVE FOR COCAINE, TETRAHYDROCANNABINO (THC), PHENOBARBITAL, PROPOXYPHENE, PENTOBARBITAL, AND MERPERIDINE RESPECTIVELY. STANDARD CURVES FOR MORPHINE, PENTOBARBITAL, PHENOBARBITAL, COCAINE, HYDROMORPHONE, AND THC ARE PRESENTED TOGETHER WITH A TABLE OF SPECIMEN AMOUNTS REQUIRED, SOLVENT, PERCENT SOLVENT RECOVERY, PERCENT OF EXTRACT FOR EMIT, QUANTITATION CURVE, DRUG CONCENTRATION PER MUGRAM/ML AND DRUG CONCENTRATION PER MUGRAM/100 ML. DESPITE THE PROCEDURE'S RELATIVELY HIGH COST, LIMITED APPLICABILITY, AND LACK OF STRUCTURAL SPECIFICITY, THE INDIRECT EMIT TECHNIQUE ELIMINATES LYSOZYME INTERFERENCE IN POST MORTEM SPECIMENS, ELIMINATES INTERFERENCE FROM INHIBITORS IN TOXICOLOGICAL SPECIMENS, CONCENTRATES ON THE SPECIFIC DRUG BEING ANALYZED, CONTROLS PH AND IONIC STRENGTH, REQUIRES MINIMAL TECHNICAL SKILL, IS FASTER THAN RADIOIMMUNASSAY, QUANTITATES DRUGS, AND ALLOWS FOR THE DETECTION OF CERTAIN DRUGS THAT MAY NOT BE DETECTED BY OTHER METHODS. REFERENCES ARE INCLUDED. (JAP)
Index Term(s): Blood/body fluid analysis; Drug testing; Evidence identification; Techniques; Tissue analysis
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http://www.ncjrs.gov/App/publications/abstract.aspx?ID=47273

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