skip navigation

PUBLICATIONS

Register for Latest Research

Stay Informed
Register with NCJRS to receive NCJRS's biweekly e-newsletter JUSTINFO and additional periodic emails from NCJRS and the NCJRS federal sponsors that highlight the latest research published or sponsored by the Office of Justice Programs.

NCJRS Abstract

The document referenced below is part of the NCJRS Virtual Library collection. To conduct further searches of the collection, visit the Virtual Library. See the Obtain Documents page for direction on how to access resources online, via mail, through interlibrary loans, or in a local library.

 

NCJ Number: 51791 Find in a Library
Title: DETECTION OF DRUGS IN A BLOODSTAIN - 1 DIPHENYLHYDANTOIN
Journal: JOURNAL OF FORENSIC SCIENCES  Volume:23  Issue:4  Dated:(OCTOBER 1978)  Pages:701-706
Author(s): R C SHALER; F P SMITH; C E MORTIMER
Corporate Author: American Acad of Forensic Sciences
United States of America
Date Published: 1978
Page Count: 6
Sponsoring Agency: Aerospace Corporation
Washington, DC 20024
American Acad of Forensic Sciences
Colorado Springs, CO 80901-0669
Sale Source: Aerospace Corporation
Forensic Sciences Dept
Suite 4040
955 L'Enfant Plaza, SW
Washington, DC 20024
United States of America
Language: English
Country: United States of America
Annotation: A RADIOIMMUNOASSAY TECHNIQUE WHICH SUCCESSFULLY ISOLATED DIPHENYLHYDANTOIN (DPH) FROM DRIED BLOODSTAINS, SALIVA, SEMEN, AND DRIED SALIVA STAINS IS DESCRIBED. ITS USE TO IDENTIFY OTHER DRUGS IN BODY FLUIDS IS DISCUSSED.
Abstract: THE DRUG USED IN THESE EXPERIMENTS IS GENERALLY LIMITED TO THE TREATMENT OF EPILEPSY. HOWEVER, THE PRINCIPLES DESCRIBED CAN BE USED TO DETECT OTHER DRUGS IN BLOOD AND BODY FLUIDS. THESE EXPERIMENTS TESTED THE ABILITY OF RADIOIMMUNOASSAY TO DETECT DPH IN SAMPLES OF WHOLE BLOOD, BLOOD PLASMA, RED BLOOD CELLS, DRIED BLOODSTAINS 1 WEEK OLD, DRIED BLOODSTAINS 6 MONTHS OLD, FRESH SALIVA AND SEMEN SAMPLES, AND SALIVA STAINS COLLECTED ON A CIGARETTE FILTER, PROVIDED BY AN INDIVIDUAL TAKING DILANTIN. THE TECHNIQUE IS EXPLAINED IN DETAIL, INCLUDING SAMPLE PREPARATION AND ELUTION BY DETERGENTS. CHARTS AND GRAPHS PRESENT THE LABORATORY RESULTS. RADIOIMMUNOASSAY PROVED EXTREMELY SENSITIVE, DETECTING DPH CONCENTRATIONS AS LOW AS 50 PICOGRAMS PER MILLILITER OF BLOOD. ALMOST AS MUCH DPH WAS DETECTED IN THE 6-MONTH OLD STAIN AS IN THE FRESH BLOODSTAIN. ALTHOUGH THERE IS SIGNIFICANTLY LESS DRUG CONCENTRATION IN SALIVA AND SEMINAL FLUID, THE DPH WAS RELIABLY DETECTED IN 0.9 MICROLITER OF UNDILUTED SAMPLE. TO MAKE THE TECHNIQUE MORE EFFECTIVE, THE RESEARCH TESTED METHODS FOR EXTRACTING GREATER QUANTITIES OF THE DRUG FROM STAINS WITH GREATER EFFICIENCY AND SHORTER INCUBATION TIMES. IT WAS FOUND THAT THE NONIONIC DETERGENT TRITON X-100 EXTRACTED ABOUT 80 PERCENT OF THE DRUG AT 1 HOUR OF INCUBATION, WHILE THE ANIONIC SODIUM DODECYL SULFATE (SDS) DETERGENT EXTRACTED 100 PERCENT OF THE AVAILABLE DRUG AT 1-HOUR OF INCUBATION. THE SALIVA STAINS ON THE CIGARETTE FILTER WERE SUCCESSFULLY EXTRACTED WITH SDS DETERGENT. INTERESTINGLY, THE FILTER YIELDED MORE THAN THE OUTSIDE FILTER PAPER. THE PRINCIPLE OF RADIOIMMUNOASSAY IS EXPLAINED. THE POSSIBILITIES OF THIS TECHNIQUE FOR FORENSIC SCIENCE ARE DISCUSSED. REFERENCES ARE INCLUDED. (GLR)
Index Term(s): Blood/body fluid analysis; Crime laboratories; Drug testing; Forensic medicine
Note: PRESENTED AT THE 29TH ANNUAL MEETING OF THE AMERICAN ACADEMY OF FORENSIC SCIENCES, SAN DIEGO, CALIF., FEBRUARY 1977
To cite this abstract, use the following link:
http://www.ncjrs.gov/App/publications/abstract.aspx?ID=51791

*A link to the full-text document is provided whenever possible. For documents not available online, a link to the publisher's website is provided. Tell us how you use the NCJRS Library and Abstracts Database - send us your feedback.