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NCJ Number: 51793 Add to Shopping cart Find in a Library
Title: GENETIC MARKERS IN HUMAN SEMEN - 2 - QUANTIFICATION OF POLYMORPHIC PROTEINS
Journal: JOURNAL OF FORENSIC SCIENCES  Volume:23  Issue:4  Dated:(OCTOBER 1978)  Pages:717-729
Author(s): E T BLAKE; G F SENSABAUGH
Corporate Author: American Acad of Forensic Sciences
United States of America
Date Published: 1978
Page Count: 13
Sponsoring Agency: American Acad of Forensic Sciences
Colorado Springs, CO 80901-0669
University of California
Berkeley, CA 94720
US Dept of Justice

US Dept of Justice NIJ Pub
Washington, DC 20531
Grant Number: 74NI-99-0041
Sale Source: University of California
School of Public Health
Forensic Sciences Group
Berkeley, CA 94720
United States of America
Language: English
Country: United States of America
Annotation: THIS PAPER DESCRIBES THE LABORATORY TECHNIQUES USED TO DETERMINE WHICH GENETIC MARKERS WERE PRESENT IN SEMEN IN SUFFICIENTLY STABLE AMOUNTS TO BE USED FOR ANALYSIS OF SEMEN EVIDENCE. COMPARISONS ARE MADE WITH BLOOD LEVELS.
Abstract: THE PREPARATION OF BLOOD AND SEMEN SAMPLES FOR ANALYSIS, THE ASSAYS USED FOR VARIOUS MARKER ENZYMES AND PROTEINS, AND THE RESULTS OF THESE ASSAYS ARE PRESENTED IN DETAIL. LABORATORY DATA SHOW THAT PHOSPHOGLUCOMUTASE, PEPTIDASE A, AND PHOSPHOGLUCOSE ISOMERASE ALL ARE PRESENT IN SEMEN AT LEVELS SUBSTANTIALLY HIGHER THAN THEIR MINIMUM QUANTITATIVE TYPING LIMIT LEVELS. THEREFORE, THERE IS NO MAJOR QUANTITATIVE BARRIER TO THEIR ANALYSIS. PHOSPHOGLUCOMUTASE HAS THE GREATEST POTENTIAL VALUE BECAUSE IT IS NOT DIFFICULT TO TYPE IN DRIED AND LIQUID SEMEN AS LONG AS THE LABORATORY TECHNICIAN IS AWARE THAT ITS PHENOTYPIC EXPRESSION IN SEMEN IS SOMEWHAT DIFFERENT FROM THAT IN BLOOD. PEPTIDASE A IS OF VALUE ONLY IN SITUATIONS INVOLVING BLACK MALES, WHILE PHOSPHOGLUCOSE ISOMERASE IS FOUND IN ONLY 1 PERCENT OF THE POPULATION AND IS OF EXTREMELY LIMITED VALUE. THOSE PROTEINS WHICH HAVE MODERATE ACTIVITY AND CAN BE TYPED UNDER FAVORABLE CIRCUMSTANCES ARE ADENYLATE KINASE, AMYLASE, ESTERASE D, GLYOXALASE, GLUCOSE-6-PHOSPHATE DEHYDROGENASE, 6-PHOSPHOGLUCONATE DEHYDROGENASE, AND TRANSFERRIN. THE USEFULNESS OF THESE SUBSTANCES IS DISCUSSED. THE MARGINALLY USEFUL MARKERS ARE ALSO IDENTIFIED. OF THESE THE IMMUNOGLOBULIN MARKERS ARE THE MOST POTENTIALLY USEFUL BUT THEIR TYPING REQUIRES EXPERTISE AND REAGENTS NOT COMMONLY AVAILABLE IN FORENSIC LABORATORIES. TABLES AND FIGURES ILLUSTRATE THE LABORATORY DATA. PROCEDURES FOR DETERMINING POLYMORPHIC PROTEIN LEVELS IN BOTH BLOOD AND SEMEN ARE DISCUSSED. THE POTENTIAL FOR EVIDENCE ANALYSIS IS EVALUATED. REFERENCES ARE PROVIDED. (GLR)
Index Term(s): Blood/body fluid analysis; Crime laboratories; Forensic medicine; Rape; Techniques; Trace evidence
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http://www.ncjrs.gov/App/publications/abstract.aspx?ID=51793

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