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NCJRS Abstract

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  NCJ Number: NCJ 227499   Add to Shopping cart   Find in a Library
  Title: Hand-Held DNA-Based Forensic Tool
  Document URL: PDF 
  Author(s): Carl A. Batt ; Scott J. Stelick ; Matthew J. Kennedy ; Clarissa S. Lui ; Adam J. Lowe
  Date Published: 2009
  Page Count: 54
  Annotation: This report presents the findings of 4 years of research and development that focused on enabling technologies for portable biosensors that can detect single nucleotide polymorphisms (SNPs), which are genetic indicators potentially useful for the development of phenotypic profiles of individuals based on field-acquired DNA samples.
  Abstract: The research anticipated that several of the technologies developed from this research - i.e., the PCR-microchip, the particle counter microchip, the molecular beacon labeling protocols, and the LDR-SERS techniques - would be integrated into a unified portable biosensor that would perform the preparation, amplification, and multiplexed optical detection of DNA. A fully portable testing platform was developed for demonstrating the PCR-based detection microchip technology. After 4 months of testing using a variety of forensic DNA samples, subsequent work developed a multiplexing instrument that featured spectral optics and multiplexed molecular labels; and two additional platforms were explored in order to achieve higher multiplexing capability. In addition, researchers developed novel microfabricated fluid pumps that are driven by low voltages and can be readily integrated into lab-on-a-chip devices. These novel pumps promise to eliminate all reagent handling duties currently performed by the end user. This work will enable portable biosensors that accept disposable cartridges preloaded with reagents. One additional platform developed was a fluorescence-activated particle counter microchip for multiplexed scoring of DNA targets. Much of the work performed focused on the use of quantum dots as molecular probes, specifically the use of quantum dots as molecular beacons. Further, the researchers developed a new approach to SNP detection through the application of Surface Enhanced Raman Scattering (SERS) to the Ligase Detection Reaction (LDR). This technique avoids the problem of spectral overlap that limit’s the number of reactions conducted in parallel by fluorescence-based systems. 33 figures, 3 tables, and an 82-item bibliography
  Main Term(s): Police policies and procedures
  Index Term(s): Crime Scene Investigation ; Crime scene ; Police equipment ; Forensics/Forensic Sciences ; DNA fingerprinting ; NIJ final report
  Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
  Grant Number: 2004-DN-BX-K001
  Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America

NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
  Type: Report (Study/Research)
  Country: United States of America
  Language: English
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