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NCJRS Abstract

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  NCJ Number: NCJ 231591   Add to Shopping cart   Find in a Library
  Title: Applications of Molecular Genetics to Human Identity
  Document URL: PDF 
  Author(s): Meredith A. Turnbough, B.S., M.S.
  Date Published: 08/2010
  Page Count: 104
  Annotation: This paper discusses methods for extracting DNA from human skeletal remains.
  Abstract: In an effort to develop more robust methods for analyzing DNA from degraded, aged, or otherwise compromised skeletal remains, this project’s objectives were to develop improved methods for extracting DNA from human skeletal remains; to improve STR profiling success of low-copy DNA samples by using whole genome amplification in order to amplify the total pool of DNA prior to STR analysis; and to improve STR profiling success of damaged DNA templates by using DNA repair enzymes in reducing the number/severity of lesions that interfere with STR profiling. Overall, bleach outperformed UV as a pretreatment, and DNA extraction using silica outperformed microconcentration and organic extraction. DNA repair with PreCRtm A outperformed both whole genome amplification and repair with PreCRtm T6. Superior DNA extraction results were achieved using the A6 PMB columns, and DNA repair with PreCRtm A led to an overall improvement in profile quality in most cases, although whole genome amplification was unsuccessful. Rapid, robust DNA isolation, successful amplification of loci from the sample-derived pool, and an elimination of DNA damage and inhibitors may assist in providing sufficient genetic information from cases that might otherwise be in the fringe of what is currently possible to obtain. 19 figures and 52 references
  Main Term(s): Criminology
  Index Term(s): Victim identification ; Suspect identification ; Bone analysis ; Forensics/Forensic Sciences ; Investigative techniques ; DNA fingerprinting ; NIJ final report ; NIJ grant-related documents
  Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
  Grant Number: 2007-DN-BX-0006
  Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America

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  Type: Report (Study/Research)
  Country: United States of America
  Language: English
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