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NCJ Number: NCJ 236825     Find in a Library
Title: Rapid Microfluidic Human Specific DNA Quantitation
Author(s): Eugene Tan
Corporate Author: NetBio Inc.
United States of America
Date Published: 2011
Page Count: 33
Sponsoring Agency: National Institute of Justice
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2008-DN-BX-K009
Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America

NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
Document: PDF 
Type: Report (Study/Research)
Language: English
Country: United States of America
Annotation: This research project developed a human-specific DNA quantitation module for incorporation into NetBio’s fully integrated microfluidic instrument and biochip, which are in development.
Abstract: The project described in this report is part of NetBio’s application of microfluidic technology for the purpose of developing an STR analysis instrument that will perform DNA purification, multiplexed PCR amplification, and electrophoretic separation and detection. This fully integrated biochip and instrument will generate STR profiles in 45 minutes without user intervention. The research described in the current report consisted of three elements: the selection and optimization of a DNA quantitation assay; the design and development of instrumentation and microfluidic biochip of DNA quantitation; and the testing of the microfluidic quantitation system using forensic samples. The implementation of this research led to the development of microfluidic biochip endpoint PCR assays. They were evaluated based on assay performance - including time to completion, sensitivity, reproducibility, and dynamic range - ease of implementation in a microfluidic format, and costs. These results constitute a significant step in the development of a fully integrated STR instrument that can have widespread use in forensic laboratories. The microfluidic DNA quantitation system will be easy to operate and compatible with both forensic and microfluidic requirements. The dissemination strategy for the research results is described. 12 figures, 1 table, and 38 references
Main Term(s): Police policies and procedures
Index Term(s): Crime Laboratories (Crime Labs) ; Victim identification ; Suspect identification ; Efficiency ; Productivity ; Investigative techniques ; DNA fingerprinting ; NIJ final report
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=258845

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