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NCJ Number: NCJ 237764   Add to Shopping cart   Find in a Library
Title: More Efficient Means to Collect and Process Reference DNA Samples
  Document URL: PDF 
Author(s): J. D. Lindstrom
Date Published: 02/2012
Page Count: 94
  Annotation: This research developed improvements in DNA processing that will enable any forensic laboratory, regardless of size, to increase its current efficiency, which will better prepare it to handle an increase in sample submissions without creating a backlog.
Abstract: This research developed a new DNA buccal collection kit that is universal in use and provides a higher success rate on the first analysis attempt. The kit combines the best qualities of several collection kits into one. The kit is simple to use and provides reliable results at an affordable cost. The kit’s direct DNA collection method helps to ensure that sufficient DNA is present, allowing the laboratory to obtain a full DNA profile on the first analysis attempt. Researchers also tested many different techniques that would allow for the direct amplification of buccal swabs. These techniques involved taking an entire swab head, lysing in various reagents, and using an aliquot of the liquid lysate for amplification. This effort demonstrated that direct amplification of buccal swabs is possible, thus eliminating the time and cost associated with the extraction step of the DNA analysis process. Researchers recommend that additional studies be conducted to find the exact combination of lysing reagents, lysing temperatures, and lysing times that will allow direct amplification from swabs. Detailed suggestions are offered for such research. 9 tables, 4 references, and appended supplementary data and information
Main Term(s): Criminology
Index Term(s): Crime Laboratories (Crime Labs) ; Evidence collection ; Victim identification ; Suspect identification ; Efficiency ; Crime laboratory management ; DNA fingerprinting ; NIJ final report
Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2009-DN-BX-K160
Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America

NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
Type: Report (Study/Research)
Country: United States of America
Language: English
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