skip navigation

Add your conference to our Justice Events calendar


Register for Latest Research

Stay Informed
Register with NCJRS to receive NCJRS's biweekly e-newsletter JUSTINFO and additional periodic emails from NCJRS and the NCJRS federal sponsors that highlight the latest research published or sponsored by the Office of Justice Programs.

NCJRS Abstract

The document referenced below is part of the NCJRS Library collection. To conduct further searches of the collection, visit the NCJRS Abstracts Database. See the Obtain Documents page for direction on how to access resources online, via mail, through interlibrary loans, or in a local library.

  NCJ Number: NCJ 241348   Add to Shopping cart   Find in a Library
  Title: NIJ Proposal to Enhance Methods for Studying Degraded DNA, Final Technical Report
  Document URL: PDF (Full Report) PDF (Appendix A) PDF (Appendix B) PDF (Appendix C) PDF (Appendix D) PDF (Appendix E) PDF (Appendix F) PDF (Appendix G) PDF (Appendix H) 
  Author(s): Brian M. Kemp ; Jodi Lynn Barta ; Kelli Flanigan ; Colin Grier ; Cara Monroe ; Justin E. Teisberg ; Misa Winters
  Date Published: 2012
  Page Count: 165
  Annotation: This final National Institute of Justice (NIJ) technical report presents the results of research that examined a proposal for improving methods for studying degraded DNA.
  Abstract: Findings from the research on the proposed method for studying degraded DNA include the following: while sodium hypochlorite was found to be highly efficient (81-99 percent) in removing contaminating DNA from bone surfaces, it did not remove 100 percent of the contamination across all experiments; and sodium hypochlorite was not found to damage endogenous ancient DNA molecules, as previous research has suggested, due to the high variability of mitochondrial DNA (mtDNA) preservation in bone samples. The primary purpose of this study was to validate methods aimed at improving reliability of the analysis of degraded skeletal samples. The research was divided into three phases: 1) determining the efficacy of using sodium hypochlorite in contamination removal from ancient skeletal specimens; 2) evaluation of the overall effectiveness of nine different thermo-stable polymerases and polymerase blends in their ability to amplify mtDNA present in skeletal samples; and 3) evaluating the extent of post-mortem DNA damage in degraded and ancient skeletal samples. Detailed information is presented on the results of each phase of the study. In addition, summaries of two additional studies are included in the report: To Clone or Not to Clone: Method Analysis for Retrieving Consensus Sequences in Ancient DNA Samples; and Cloning May Not Be a Necessary Criterion for the Authentication of Ancient DNA Consensus Sequences and Damage Appears to be Randomly Distributed Across the Human Mitochondrial HVRI Region. Tables, figures, and references
  Main Term(s): Forensics/Forensic Sciences
  Index Term(s): Evidence identification and analysis ; Evidence preservation ; Analysis ; Bone analysis ; NIJ final report ; NIJ grant-related documents
  Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
  Grant Number: 2008-DN-BX-K008
  Sale Source: NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
  Type: Report (Technical)
  Country: United States of America
  Language: English
  To cite this abstract, use the following link:

*A link to the full-text document is provided whenever possible. For documents not available online, a link to the publisher's website is provided. Tell us how you use the NCJRS Library and Abstracts Database - send us your feedback.