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NCJ Number: NCJ 241912   Add to Shopping cart   Find in a Library
Title: Identification and Separation of Evidence Mixtures Using SNP-Based FISH Techniques and Laser Microdissection
  Document URL: PDF 
Author(s): Abigail Bathrick, M.F.S. ; Jared Latiolais, M.S., M.F.S. ; Robert Bever, Ph.D.
Date Published: 07/2012
Page Count: 87
  Annotation: This study examined the feasibility of separating cellular mixtures of the same morphology and gender by developing fluorescence in situ hybridization(FISH) probes based on human genetic single nucleotide polymorphisms (SNPs).
Abstract: Findings of this research indicated that SNPs are not ideal targets for forensic FISH probing. Probe signals specific to the desired SNP were not detected. It is unknown if this was caused by the specificity of the probe hybridization or the sensitivity of the detection protocols; either is a plausible explanation for the lack of signals. Failure to successfully hybridize to the target sequence and successful hybridization with insufficient detection sensitivity would both produce negative results. Unsuccessful hybridization can be addressed by redesigning the probe or optimizing the hybridization conditions; however, it can be challenging and time consuming to identify the cause of the issue. Examination of alternative RCA techniques may improve the detection sensitivity; however, the alternative RCA techniques may not possess the efficiency necessary for forensic use. It has been reported that RCA is successful in only 20 percent to 55 percent of interphase nuclei targets. For example, if 200 cells are present on a slide, as few as 40 would generate signals. Cells that are not successfully identified with FISH probing cannot be collected for further analysis and key evidence material may be left on the slide. Based on these results, it is recommended to focus future efforts on genetic marker systems that consist of larger genetic differences. Tables, figures, and references
Main Term(s): DNA fingerprinting
Index Term(s): Research methods ; Research and development ; Research design ; Research uses in policymaking ; Forensics/Forensic Sciences ; Criminal justice research ; NIJ final report ; NIJ grant-related documents
Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2009-DN-BX-K250
Sale Source: NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
Type: Report (Technical)
Country: United States of America
Language: English
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