skip navigation

CrimeSolutions.gov

Add your conference to our Justice Events calendar

PUBLICATIONS

Register for Latest Research

Stay Informed
Register with NCJRS to receive NCJRS's biweekly e-newsletter JUSTINFO and additional periodic emails from NCJRS and the NCJRS federal sponsors that highlight the latest research published or sponsored by the Office of Justice Programs.

NCJRS Abstract

The document referenced below is part of the NCJRS Library collection. To conduct further searches of the collection, visit the NCJRS Abstracts Database. See the Obtain Documents page for direction on how to access resources online, via mail, through interlibrary loans, or in a local library.

 
  NCJ Number: NCJ 247083     Find in a Library
  Title: Sperm Capture Using Aptamer Based Technology
  Document URL: PDF 
  Author(s): Evaldas Katilius ; Glenn Sanders ; Andrew Carmel ; Larry Gold ; Greggory S. LaBerge
  Date Published: 06/2014
  Page Count: 42
  Annotation: The Denver Police Department Crime Laboratory, in collaboration with SomaLoci, Inc. (Boulder, CO), identified and developed a DNA aptamer-based system for sperm-binding, isolation, and purification for DNA analysis in sexual assault cases.
  Abstract: This is the first aptamer-based system used in whole cell sperm captures and adapted for use with forensic casework samples. Subsequent short tandem repeat (STR) DNA analysis showed clean male DNA profiles from sperm cells eluted from cotton swabs, the preferred method for rape kit evidence collection. SOMA-based sperm cell separation is comparable to existing differential extraction-based methods; however, more rapid sample processing allows for sperm purification and DNA extraction to be completed in significantly less time. Over the last several years, a class of DNA aptamer molecules called SOMAmers (Slow Off-rate Modified Aptamers) has been identified; they bind specifically to sperm heads and tails. Several SOMAmer candidates have been further selected with the ability to bind sperm under very stringent and selective conditions. Conditions that support both efficient sperm cell elution from cotton swabs and capture using SOMAmer-coated magnetic beads were identified by screening numerous combinations of detergents, salts, and buffer additives. When tethered to magnetic beads using Biotin and Streptavidin linkers, selected SOMAmers demonstrated efficient affinity-based purification of sperm cells from eluates of mock forensic samples that contained a mixture of HeLa cells and sperm cells or female buccal cells and sperm cells. Further research is ongoing in optimizing these SOMAmers under various stringent and forensically relevant conditions, so as to develop an inexpensive and rapid system for sperm cell capture adapted for automation, using robotic workstations commonly found in forensic laboratories. A full forensic validation will be conducted to address reproducibility, sensitivity, and reliability with both mock and adjudicated forensic case samples. 8 figures, 1 table, and 20 references
  Main Term(s): Criminology
  Index Term(s): Suspect identification ; Sexual assault ; Rape investigations ; Forensics/Forensic Sciences ; Investigative techniques ; DNA fingerprinting ; DNA Typing ; DNA extraction ; Mixed DNA profiles
  Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
  Grant Number: 2009-DN-BX-K403
  Sale Source: NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
  Type: Report (Study/Research) ; Research (Applied/Empirical) ; Report (Grant Sponsored)
  Country: United States of America
  Language: English
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=269182

*A link to the full-text document is provided whenever possible. For documents not available online, a link to the publisher's website is provided. Tell us how you use the NCJRS Library and Abstracts Database - send us your feedback.