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NCJRS Abstract

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NCJ Number: 136875 Find in a Library
Title: Deoxyribonucleic Acid (DNA) Typing of Human Leukocyte Antigen (HLA)-DQA1 from Single Hairs in Japanese
Journal: Journal of Forensic Sciences  Volume:37  Issue:3  Dated:(May 1992)  Pages:853-859
Author(s): R Uchihi; K Tamaki; T Kojima; T Yamamoto; Y Katsumata
Date Published: 1992
Page Count: 7
Type: Report (Technical)
Format: Article
Language: English
Country: United States of America
Annotation: Recent advances in recombinant deoxyribonucleic acid (DNA) techniques using a variable number of tandem repeat probes or minisatellite probes have changed current forensic procedures and brought about new applications in forensic science practice.
Abstract: In particular, DNA typing of human leukocyte antigen (HLA)-DQA1 from single hairs is described, based on an analysis of freshly plucked hair samples obtained from 12 healthy Japanese. Hairs were separated into root and shaft portions, and DNA was extracted according to the protocol described by Higuchi et al. The specific region of the HLA-DQA1 gene was amplified by polymerase chain reaction (PCR). It was not easy, however, to type HLA-DQA1 with hair shaft portions. An increase in the specimens of hair shaft portions over 10 centimeters in length to obtain sufficient DNA inhibited the PCR. Synthetic melanin, as well as the one extracted from hairs, inhibited the PCR of the genomic DNA template when added to the PCR reaction at a concentration over 15 ng/100 micro L. The typability of hair shaft portions seemed to depend on the delicate balance of DNA concentrations and contaminated melanin in final DNA extracts. 22 references, 2 tables, and 3 figures (Author abstract modified)
Main Term(s): Hair and fiber analysis
Index Term(s): Blood/body fluid analysis; Criminal investigation; Forensic medicine; Japan
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