The sample was divided into four groups, with CF specimens taken 2, 4, 10, and 24 hours after death. The results showed that the enzyme activity of CK and CK-BB increased linearly and statistically significantly 4 to 24 hours postmortem. The 2-hour value was 10 to 20 times higher than in vivo, LD and its isoenzymes 1 to 3 distinctly 10 to 24 hours after death. Glucose and pyruvate concentrations in the CF declined as did Na+ and Cl-. Lactate and K+ increased over time. The earliest statistically significant changes between different timepoints were seen in lactate, pyruvate, and K+ concentrations. The GABA concentration was more than 170 times at 2 hours postmortem, and glutamate more than 20 times higher than in vivo. Concentrations of alanine, glycine, lysine, histidine, isoleucine, phenylalanine, and tyrosine were two to three times higher at 2 hours postmortem than in vivo. Concentrations of ammonia and all amino acids increased linearly and statistically significantly in the CF 4 to 24 hours postmortem. 2 tables, 5 figures, and 52 references