The Luminol test is routinely used in forensic serology to locate blood traces and identify blood stains not visible to the naked eye; its sensitivity is reported as ranging from 1:100,000 to 1:5,000,000. To evaluate the possibility of correlating the post-mortem interval with blood remnants in bone tissue, the Luminol test was performed on 80 femurs with a known time of death, grouped in five classes. Powdered bone (30 mg) was recovered from compact tissue of the mid-shaft of each femur and was treated with 0.1 mL of Luminol solution. The reactions were observed in a dark room and filmed by a television camera equipped with a recording tape. An intense chemiluminescence was observed after a few seconds in all femurs with a PMI ranging from 1 month to 3 years. On the 20 femurs with a PMI of 10-15 years, a clear chemiluminescence was visible with the naked eye in 80 percent of the sample. Among the 20 femurs with a PMI of 25-35 years, a weaker chemiluminescence appeared in 7 femurs. In femurs with a PMI of 50-60 years, a faint reaction was observed in only a single femur. Chemiluminescence was not observed in any of 10 femurs with a PMI over 80 years. Tables, references