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Intentional Mixed Buccal Cell Reference Sample in a Paternity Case

NCJ Number
217657
Journal
Journal of Forensic Sciences Volume: 52 Issue: 2 Dated: March 2007 Pages: 397-399
Author(s)
Luis J. Martinez-Gonzalez M.S.; Jose A. Lorente Ph.D.; Esther Martinez-Espin M.S.; J. Carlos Alvarez Ph.D.; Miguel Lorente Ph.D.; Enrique Villanueva Ph.D.; Bruce Budowle Ph.D.
Date Published
March 2007
Length
3 pages
Annotation
This paper reports on the resolution of a case in which an alleged father (AF) involved in paternity testing introduced someone else's saliva into his mouth in an effort to manipulate the buccal swab sample used in the DNA analysis, so as to exclude himself as the father.
Abstract
The first indication of a potential problem in the testing was the presence of an atypical profile of the AF, which showed evidence of a mixture of DNA (contamination). The DNA profile from the AF showed extra peaks in some loci as well as a much higher "X" allele peak relative to the "Y" allele peak at the amelogenin locus. After conducting a reanalysis by another technician with another set of positive and negative controls, analysts concluded that the only source of the mixed profile was the intentional introduction of some foreign human biological material by the AF at the point of sampling. When the AF was called back to the lab and presented with an explanation and conclusions of the findings, he admitted introducing the foreign saliva in an attempt to be excluded as the father of the child. Following this case, the sampling protocol for donors was changed. Now a staff member is always with the donor, including during the rinsing of his mouth. 2 figures, 1 table, and 4 references