The results of independence testing support the use of these data for estimating the occurrence/rarity of a DNA profile containing these two short tandem repeat (STR) loci, D2S1338 and D19S433, for the five identified racial groups. Findings revealed that both loci were highly polymorphic in all five sample populations. The research method involved the use of the new multiplex kit, the AmpFISTR Identifier PCR Amplification Kit, which was developed to enable simultaneous amplification of the 13 core CODIS STR loci, 2 additional STR loci (D2S1338 and D19S433), and the amelogenin. The DNA samples were amplified and then separated by capillary electrophoresis using an ABI Prism 3700 DNA Analyzer and separation medium POP 6 Performance Optimizer Polymer. Allele designations were determined by comparison of the amplicon fragment with those of the allelic ladders and internal lane standards using Applied Biosystems Genotyper software. The statistical analysis involved calculating the frequency of each allele for each locus from the numbers of each genotype in the sample set, known as the gene count method. A Web site address is provided for the genotype profile data used for this study. References