A partial STR profile was obtained from DNA that was recovered from an archived membrane using conventional extraction buffer components, indicating promise for recovering useful STR information from RFLP membranes that have been maintained in long-term frozen storage. This raises the possibility of recovering DNA from archived membranes and generating STR profiles that could be compared directly to suspect/victim standards or possibly entered into CODIS for database comparison. Although modified versions of casework stain extraction buffer may be sufficient for recovering amplifiable template, more vigorous methods may be needed, depending on the materials and protocols used for the RFLP analysis and the nature of the storage conditions thereafter. The studies investigated several methods for obtaining STR profiles from membrane-bound DNA, including removal of bound DNA with bases, acids, detergents, various chemicals, and conventional cell extraction solutions. Direct multiplex STR amplification of template in the membrane-bound state was also explored. In addition to detailed descriptions of the various methods used in an effort to obtain STR profiles from membrane-bound DNA, this report describes DNA extraction, quantitation, amplification, and analysis; STR amplification of HaeIII-restricted DNA and amplicon sequence analysis, and binding DNA to nylon membrane. 3 tables, 1 figure, and 35 references