The study found a means of unifying two tests, one for identifying urine as of human origin and a second for DNA typing from small stains of human urine, for use on just one sample. A urine stain was extracted with phosphate-buffered saline, and the extract was filtered using a Centricon-100 device. The filtrate was subjected to electrospray ionization liquid chromatography-mass spectrometry for identification of human urine, and a DNA-typing sample was obtained by dialfiltration of the residue, using a DNA purification kit. After the purified residue was treated with an AmpflSTR Profiler PCR amplification kit, the DNA-types were analyzed by capillary electrophoresis using a Genetic analyzer. This procedure was successfully applied in a murder case that involved a urine stain found on a waterproof sheet in a motel bedroom. Detailed descriptions of materials and methods address the reagents used, standard solutions, sample characteristics and preparation, the extraction procedure, conditions of high-performance liquid chromatography/mass spectrography, and amplification and electrophoresis of DNA. 1 table, 6 figures, and 4 references