NCJ Number
223395
Journal
Journal of Forensic Sciences Volume: 53 Issue: 3 Dated: May 2008 Pages: 683-686
Date Published
May 2008
Length
4 pages
Annotation
Although polymorphism of locus D17S2266E--a new, variable genetic marker that exhibits polymorphism of the number of repeats of four-nucleotide and two-nucleotide motifs--has been known for a few years, proper genetic analysis and forensic application are presented in this report.
Abstract
The biostatistical parameters show that the marker D17S2266E has a wide range of applications in forensic testing. This study, which was conducted with 250 unrelated persons from various regions of Poland, identified 24 different alleles that ranged in size from 232 to 290 base pairs. Analysis of the sequenced fragments showed that the alleles consisted of two flanking regions and two variable blocks that were separated by a consensus sequence. There were (AAAG)5(AG)1(AAG)3-4 repeats in the first block and [(AAAG)2(AG)1]0-1[(AAAG)6(AG)1]0-1(AAAG)n repeats in the second block. On the basis of the allele frequencies in the population, researchers were able to do biostatistical calculation, which yielded the following results: expected heterozygosity 0.8947 plus or minus 0.0137, power of discrimination 0.9793, polymorphism information content 0.8837, probability of exclusion (PE) 0.7859, PE for motherless cases 0.6473, and an average paternity index of 4.7470. 3 tables and 28 references