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NCJRS Abstract

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  NCJ Number: NCJ 236691     Find in a Library
  Title: Isolation of Highly Specific Protein Markers for the Identification of Biological Stains: Adapting Comparative Proteomics to Forensics
  Document URL: PDF 
  Author(s): Phillip B. Danielson Ph.D.
  Date Published: 2011
  Page Count: 38
  Annotation: This study tested the hypothesis that by rigorously comparing the “proteomic profiles” (proteins present in a substance) of six body fluids of particular value to the forensic community, a panel of high-specificity candidate protein biomarkers for individual body fluids could be identified.
  Abstract: The circumstance that warranted this project is the fact that although DNA profiling allows biological stains to be individualized, the unambiguous identification of the stain itself can present forensic serologists with a significant challenge; for example, there is no reliable test for vaginal secretions, and tests for blood cannot distinguish peripheral from menstrual blood. Such information can be probative to an investigation. This research confirmed the hypothesis. Through a combination of highly reproducible 2-Dimensional High-Performance Liquid Chromatography (2 D HPLC) proteome fractionation and implementation of customized clustering algorithms, high-resolution consensus proteomic profiles (2D pl/hydrophobicity maps) were produced for each of six different bodily fluids (saliva. semen, peripheral blood, menstrual blood, vaginal secretions, and urine). By conducting quantitative pair-wise comparisons among these datasets, it was possible to distinguish those proteins that were likely to be genuinely characteristic of a specific bodily fluid versus those that reflected inter-individual variability in protein expression. False negatives were minimized by normalizing the consensus datasets for subtle variations in pH, solvent gradients, and protein concentration. Based on these analyses, a comprehensive panel of candidate biomarkers was produced and characterized by mass spectrometry. A thorough validation of the specificity of these candidate biomarkers in a larger population group, as well as using forensic casework type samples, are the next steps in the development of a practitioners-ready high-specificity test for biological stain identification. 1 table, 11 figures, 28 references, and a listing of talks and posters on the research findings
  Main Term(s): Police policies and procedures
  Index Term(s): Trace evidence ; Evidence identification and analysis ; Blood/body fluid analysis ; Forensics/Forensic Sciences ; Blood stains ; NIJ final report
  Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
  Grant Number: 2006-DN-BX-K001
  Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America

NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
  Type: Report (Study/Research)
  Country: United States of America
  Language: English
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=258706

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