Narrated by Raymond Burr, the film shows how a 'one time too often' drink of moonshine from a roadside restaurant results in a death from poisoning of a young woman's fiance. The film also shows how Treasury agents follow up on the death by obtaining a sample of the moonshine and subjecting it to neutron activation analysis in order to trace the illegal moonshine still. In addition, the film shows some characteristics of an illegal moonshine operation, a raid on the still by two Treasury agents, the successful capture of the still operators, and the closing down of the roadside moonshine distributor.