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NCJ Number: 249281 Find in a Library
Title: Metagenomic Assessment of the Bacteria Associated With Lucilia Sericata and Lucilia Cuprina (Diptera: Calliphoridae)
Journal: Applied Microbiology and Biotechnology  Volume:99  Issue:2  Dated:January 2015  Pages:869-883
Author(s): Baneshwar Singh; Twani L. Crippen; Longyu Zheng; Andrew T. Fields; Zinin Yu; Qun Ma; Thomas K. Wood; Scot E. Dowd; Micah Flores; Jeffery K Tomberlin; Aaron M. Tarone
Date Published: January 2015
Page Count: 15
Sponsoring Agency: National Institute of Justice (NIJ)
Washington, DC 20531
Grant Number: 2010-DN-BX-K243
Document: HTML
Type: Report (Grant Sponsored); Report (Study/Research); Research (Applied/Empirical)
Format: Article; Document (Online)
Language: English
Country: United States of America
Annotation: This study characterized bacteria associated with different life stages of Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) and in the salivary gland of L. sericata by using 16S rDNA 454 pyrosequencing. Bacteria associated with the salivary gland of L. sericata were also characterized using light and transmission electron microscopy (TEM).
Abstract: Overall, this study provides information on bacterial communities associated with different life stages of Lucilia and their horizontal and trans-generational transmission, which may help in the development of better vector-borne disease management and MDT methods. Lucilia Robineau-Desvoidy (Diptera: Calliphoridae) is a blow fly genus of forensic, medical, veterinary, and agricultural importance. This genus is also famous because of its beneficial uses in maggot debridement therapy (MDT). Although the genus is of considerable economic importance, our knowledge about microbes associated with these flies and how these bacteria are horizontally and trans-generationally transmitted is limited. Results from the current study suggest that the majority of bacteria associated with these flies belong to phyla Proteobacteria, Firmicutes, and Bacteroidetes, and most bacteria are maintained intragenerationally, with a considerable degree of turnover from generation to generation. In both species, second-generation eggs exhibited the highest bacterial phylum diversity (20-percent genetic distance) than other life stages. The Lucilia sister species shared the majority of their classified genera. Of the shared bacterial genera, Providencia, Ignatzschineria, Lactobacillus, Lactococcus, Vagococcus, Morganella, and Myroides were present at relatively high abundances. Lactobacillus, Proteus, Diaphorobacter, and Morganella were the dominant bacterial genera associated with a survey of the salivary gland of L. sericata. TEM analysis showed a sparse distribution of both Gram-positive and Gram-negative bacteria in the salivary gland of L. sericata. There was more evidence for horizontal transmission of bacteria than there was for trans-generational inheritance. Several pathogenic genera were either amplified or reduced by the larval feeding on decomposing liver as a resource. (Publisher abstract modified)
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Death investigations; Investigative techniques; NIJ grant-related documents; Time of death determination
To cite this abstract, use the following link:
http://www.ncjrs.gov/App/publications/abstract.aspx?ID=271425

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