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NCJ Number: 249371 Find in a Library
Title: Instrument Parameters Controlling Retention Precision in Gradient Elution Reversed-phase Liquid Chromatography
Journal: Journal of Chromatography A  Volume:1371  Dated:December 2014  Pages:90-105
Author(s): Ayse Beyaz; Wenzhe Farr; Peter Carr; Adam P. Schellinger
Date Published: December 2014
Page Count: 16
Sponsoring Agency: National Institute of Justice (NIJ)
Washington, DC 20531
Document: HTML
Type: Report (Grant Sponsored); Report (Study/Research); Research (Applied/Empirical)
Format: Article; Document (Online)
Language: English
Country: United States of America
Annotation: This study examined the effect of temperature (T), initial (ϕo) and final mobile phase (ϕf) composition, gradient time (tG), and flow rate (F ) on the retention time precision under gradient elution conditions for various types of low MW solutes.
Abstract: The study determined the retention factor in pure water (k'wk'w) and the solute-dependent solvent strength (S) parameters of Snyder's linear solvent strength theory (LSST) as a function of temperature for three different groups of solutes. The effect of small changes in the chromatographic variables (T, ϕo, ϕf, tG and F) by use of the LSST gradient retention equation were estimated. Peaks at different positions in the chromatogram have different sensitivities to changes in these instrument parameters. In general, absolute fluctuations in retention time are larger at longer gradient times. Drugs showed less sensitivity to changes in temperature compared to relatively less polar solutes, non-ionogenic solutes. Surprisingly, results showed that fluctuations in temperature, mobile phase composition, and flow rate had less effect on retention time under gradient conditions compared to isocratic conditions. Overall temperature and the initial mobile phase composition are the most important variables affecting retention reproducibility in gradient elution chromatography. (Publisher abstract modified)
Main Term(s): Forensic sciences
Index Term(s): Chromatography; Crime laboratory equipment; Drug analysis; NIJ grant-related documents; NIJ Resources
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