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NCJRS Abstract

The document referenced below is part of the NCJRS Library collection. To conduct further searches of the collection, visit the NCJRS Abstracts Database. See the Obtain Documents page for direction on how to access resources online, via mail, through interlibrary loans, or in a local library.

 
  NCJ Number: NCJ 217267     Find in a Library
  Title: Development of Microdevice Solid-Phase Purification Utilizing Dual Pressure/Electro-Elution for Concentration and Enhanced Recovery of DNA
  Document URL: PDF 
  Author(s): Joan Bienvenue
  Date Published: 02/2007
  Page Count: 23
  Annotation: This paper describes the evaluation of a device that uses an electric field during the DNA elution stage of the solid phase extraction process in order to enhance recovery of DNA and provide a more concentrated sample for subsequent genetic analysis.
  Abstract: The evaluation demonstrated the application of an electric field while retaining and capturing DNA with precision elution and maintaining solution flow through the device designed for this evaluation. This was the first example of electrophoretically retained DNA during the course of micro-solid phase purification. It is the first step toward addressing the volume incompatibility between the DNA purification and PCR amplification domains in integrated microfluidic devices. The paper advises, however, that further development of the device design and protocol is required in order to ensure that the majority of the eluting DNA is retained. The preliminary results of this evaluation suggest that with proper evaluation and continued design development, the complete capture, concentration, and retention of DNA can be achieved. This paper describes the preliminary design for a glass microdevice capable of electro-solid phase extraction and dual pressure/electro-elution. Using this device, a typical solid phase extraction (sample load, protein wash, and DNA elutions) was performed using pressure-driven flow. The electric field was imposed during the final elution step in order to trap DNA as it exited the device. 12 figures and 24 references
  Main Term(s): Criminology
  Index Term(s): Technology transfer ; Science and Technology ; Forensics/Forensic Sciences ; Investigative techniques ; DNA fingerprinting ; NIJ final report ; NIJ grant-related documents
  Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
  Grant Number: 2005-DA-BX-K100
  Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America

NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
  Type: Report (Study/Research) ; Test/Measurement
  Country: United States of America
  Language: English
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=238894

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